Product Info Summary
SKU: | LS054821 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of excision repair cross-complementing rodent repair deficiency, complementation group 6-like (ERCC6L) |
Product info
Product Name
ERCC6L (NM_017669) Human Over-expression Lysate
SKU/Catalog Number
LS054821
Size
100 µg
Description
Transient overexpression lysate of excision repair cross-complementing rodent repair deficiency, complementation group 6-like (ERCC6L)
Storage & Handling
Cite This Product
ERCC6L (NM_017669) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054821)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
ERCC6L Human Over-expression Lysates NM_017669.
Western blot validation of overexpression lysate (LS054821) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ERCC6L (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ERCC6L (Source: Uniprot.org, NCBI)
Gene Name
ERCC6L
Full Name
DNA excision repair protein ERCC-6-like
Weight
141.103kDa
Superfamily
SNF2/RAD54 helicase family
Alternative Names
ATP-dependent helicase ERCC6-like; DNA excision repair protein ERCC-6-like; EC 3.6.1; EC 3.6.4.12; excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like; excision repair protein ERCC6-like; FLJ20105; MGC131695; PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like; PLK1-interacting checkpoint helicase; SNF2/RAD54 family protein; Tumor antigen BJ-HCC-15 ERCC6L PICH, RAD26L ERCC excision repair 6 like, spindle assembly checkpoint helicase DNA excision repair protein ERCC-6-like|ATP-dependent helicase ERCC6-like|Plk1-interacting checkpoint helicase|SNF2/RAD54 family protein|excision repair cross-complementation group 6 like|excision repair cross-complementing rodent repair deficiency complementation group 6 - like|excision repair cross-complementing rodent repair deficiency, complementation group 6-like|excision repair protein ERCC6-like|tumor antigen BJ-HCC-15
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ERCC6L, check out the ERCC6L Infographic
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In this infographic, you will see the following information for ERCC6L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ERCC6L (NM_017669) Human Over-expression Lysate (LS054821)
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